Cal-590 Potassium Salt:低背景红色荧光适配多色成像的钙离子指示剂 99xcs.com【基本概念】

Cal-590 Potassium Salt在保留Rhod-2光谱波长的基础上,优化了细胞装载效率与钙离子响应性能,同时可适配TRITC和 Cy3滤光片组。

英文名称:Cal-590 Potassium Salt;Cal-590钾盐

中文名称:Cal-590 Potassium Salt钙离子荧光探针

分子量:1123.03

Ex(nm):574

Em(nm):588

溶剂:DMSO

纯度:95%+

性状:固体

用途:仅用作科研实验

规格包装:常规包装为mg级,可根据需求定制

储存温度:-20℃,同时需确保环境干燥、避光、防潮

【多种优势】

在仓鼠卵巢细胞(CHO)与人胚肾细胞(HEK)中,Cal-590 Potassium Salt对细胞内钙离子浓度变化的响应灵敏度远高于Rhod-2 AM。Cal-590 Potassium Salt的光谱与FITC、Alexa Fluor 488以及GFP的光谱完全分离,因此它是一种理想的钙离子探针,可用于GFP细胞系,FITC或Alexa Fluor 488标记抗体的细胞内多重检测实验。

【参考文献】

1. Development of a deep two-photon calcium imaging method for the analysis of cortical processing in the mammalian brain

Authors: Birkner, Antje

2. A versatile and Open-Source rapid LED switching system for One-Photon imaging and Photo-Activation

Authors: Battefeld, Arne and Popovic, Marko A and Van der Werf, Dirk and Kole, Maarten HP

Journal: Frontiers in cellular neuroscience (2019): 530

3. Gap junction coupling shapes the encoding of light in the developing retina

Authors: Caval-Holme, Franklin and Zhang, Yizhen and Feller, Marla B

Journal: Current Biology (2019): 4024--4035

【相关试剂】

DOTA-DBCO

DOTATATE

DOTAGA2

DOTA-Maleimide

DOTA-Tetrazine

DOTA-MeTz

DOTA-PEG5-amine

DOTA-PEG3-azide

DOTA-PEG5-azide

DOTA-p-NCS-Bn

DOTA-p-NH2-Bn

DOTA-PEG4-MeTz

DOTA-PEG5-C4-DBCO

DOTAGA-(COOt-Bu)4

DOTA-PEG8-NHCO-MeTz

DOTA-PEG3-azide(HCl)

(R)-DOTAGA-(COOt-Bu)4

(S)-DOTAGA-(COOt-Bu)4

本文由【西安强化生物】编辑乐乐整理,内容仅供科研参考,不用于临床诊断。